Description

Rebaudioside D is a high-value diterpenoid glycoside isolated from the leaves of Stevia rebaudiana Bertoni. BIORLAB offers research-grade Rebaudioside D with exceptional purity (≥98%) for pharmaceutical, food science, and health product development applications. This compound represents one of the rarer, high-potency steviol glycosides with superior taste characteristics compared to many related compounds.

Technical Specifications

• Appearance: White to off-white crystalline powder
• Molecular Weight: 1129.2 g/mol
• Chemical Class: Steviol glycoside (diterpenoid glycoside)
• Purity: ≥98% (HPLC)
• Melting Point: 215-217°C (decomposes)
• Optical Rotation: [α]D20 -22° to -26° (c 1.0, methanol)
• Solubility: Soluble in water, methanol, ethanol; poorly soluble in non-polar solvents

IUPAC Name: [(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl] (1R,4S,5R,9S,10R,13S)-13-[(2S,3R,4S,5R,6R)-5-hydroxy-6-(hydroxymethyl)-3,4-bis[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]oxan-2-yl]oxy-5,9-dimethyl-14-methylidenetetracyclo[11.2.1.01,10.04,9]hexadecane-5-carboxylate

InChI: InChI=1S/C50H80O28/c1-18-11-49-9-5-24-47(2,7-4-8-48(24,3)46(68)77-44-39(34(64)29(59)22(15-54)72-44)75-42-36(66)32(62)27(57)20(13-52)70-42)25(49)6-10-50(18,17-49)78-45-40(76-43-37(67)33(63)28(58)21(14-53)71-43)38(30(60)23(16-55)73-45)74-41-35(65)31(61)26(56)19(12-51)69-41/h19-45,51-67H,1,4-17H2,2-3H3/t19-,20-,21-,22-,23-,24+,25+,26-,27-,28-,29-,30-,31+,32+,33+,34+,35-,36-,37-,38+,39-,40-,41+,42+,43+,44+,45+,47-,48-,49-,50+/m1/s1

InChIKey: RPYRMTHVSUWHSV-CUZJHZIBSA-N

Canonical SMILES: CC12CCCC(C1CCC34C2CCC(C3)(C(=C)C4)OC5C(C(C(C(O5)CO)O)OC6C(C(C(C(O6)CO)O)O)O)OC7C(C(C(C(O7)CO)O)O)O)(C)C(=O)OC8C(C(C(C(O8)CO)O)O)OC9C(C(C(C(O9)CO)O)O)O

Isomeric SMILES: C[C@@]12CCC[C@@]([C@H]1CC[C@]34[C@H]2CC[C@](C3)(C(=C)C4)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)CO)O)O)O)(C)C(=O)O[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O)O[C@H]9[C@@H]([C@H]([C@@H]([C@H](O9)CO)O)O)O

Synonyms: Rebaudioside D; 63279-13-0; (-)-Rebaudioside D; Rebaudioside D, (-)-; Rebaudioside-D; UNII-1SU114WXBX; 1SU114WXBX; RebD; …

Botanical Source: Stevia rebaudiana (Bertoni) Hemsl.

Storage and Handling Requirements

• Store in airtight containers at -20°C
• Protect from light and moisture
• Hygroscopic – handle in controlled humidity environments
• Stable for 24 months when properly stored
• Working solutions can be prepared in water or alcohol
• Allow container to reach room temperature before opening to prevent moisture condensation

Research Applications

Pharmaceutical Development

• Natural Sweetener Research: Critical for developing non-caloric sweetener systems with improved taste profiles
• Diabetes Management Studies: Valuable for research on non-glycemic sweeteners for diabetic formulations
• Glycoside Stability Research: Important for investigating stability in various pH environments and formulation conditions
• Structure-Activity Relationship Studies: Essential for understanding sweetness perception mechanisms

Food Science Applications

• Flavor Modulation Research: Significant potential for reducing bitterness and aftertaste in food systems
• Sweetener Synergy Studies: Important for investigating synergistic effects with other sweeteners
• Heat Stability Evaluations: Critical for research on baking and cooking applications
• Formulation Science: Valuable for developing sugar-reduced food products

Health Products Development

• Natural Product Research: Essential component for developing clean-label sweetener systems
• Functional Food Studies: Important for research on bioactive properties beyond sweetness
• Dental Health Research: Valuable for studies on non-cariogenic sweetener systems
• Metabolic Response Investigations: Critical for understanding physiological responses to non-nutritive sweeteners

Analytical Profile

• Chromatographic Profile: Characterized by HPLC retention time of 9.8-10.2 minutes (under standard conditions)
• UV Absorption: λmax at 210 nm
• Mass Spectrum: Molecular ion peak [M-H]- at m/z 1128.1
• NMR Fingerprint: Distinctive 1H and 13C NMR spectra confirming glycosidic linkages and steviol backbone

Quality Assurance

BIORLAB’s Rebaudioside D undergoes:

• Comprehensive chromatographic analysis (HPLC-UV, HPLC-MS)
• Structure verification by 1H and 13C NMR spectroscopy
• Heavy metal analysis (<10 ppm total heavy metals)
• Microbial testing (meets USP <61> and <62> specifications)
• Residual solvent analysis (meets ICH Q3C guidelines)
• Stability studies under various environmental conditions

Sourcing and Sustainability

• Ethically sourced from controlled Stevia rebaudiana cultivations
• Sustainable agricultural practices
• Environmentally responsible extraction processes
• Full traceability from plant to purified compound
• Standardized isolation procedures ensuring batch-to-batch consistency

Competitive Advantages

• Higher purity than market standard (≥98%)
• Superior taste profile compared to Rebaudioside A
• Comprehensive analytical documentation
• Technical consultation available
• Custom purification options
• Bulk quantities available for scale-up research

For research and development purposes only. Not for direct human consumption without appropriate regulatory approval. 

Contact BIORLAB’s technical support team for detailed specifications, bulk pricing, or custom purification requirements.