Description

High-purity Bufalin, a bioactive cardiotonic steroid from toad venom (Bufo species), specifically targeting drug-resistant cancers and chemoresistance mechanisms.

Technical Specifications

• Molecular Formula: C₂₄H₃₄O₄
• Molecular Weight: 386.5 g/mol
• Purity: ≥98% (HPLC)
• Appearance: White to off-white crystalline powder
• Solubility: Soluble in DMSO, and methanol; poorly soluble in water

IUPAC Name: 5-[(3S,5R,8R,9S,10S,13R,14S,17R)-3,14-dihydroxy-10,13-dimethyl-1,2,3,4,5,6,7,8,9,11,12,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pyran-2-one

InChI: InChI=1S/C24H34O4/c1-22-10-7-17(25)13-16(22)4-5-20-19(22)8-11-23(2)18(9-12-24(20,23)27)15-3-6-21(26)28-14-15/h3,6,14,16-20,25,27H,4-5,7-13H2,1-2H3/t16-,17+,18-,19+,20-,22+,23-,24+/m1/s1

InChIKey: QEEBRPGZBVVINN-BMPKRDENSA-N

Canonical SMILES: CC12CCC(CC1CCC3C2CCC4(C3(CCC4C5=COC(=O)C=C5)O)C)O

Isomeric SMILES: C[C@]12CC[C@@H](C[C@H]1CC[C@@H]3[C@@H]2CC[C@]4([C@@]3(CC[C@@H]4C5=COC(=O)C=C5)O)C)O

Synonyms: bufalin; 465-21-4; CHEBI:517248; 3-beta,14-Dihydroxy-5-beta-bufa-20,22-dienolide; 3,14-Dihydroxy-bufa-20,22-dienolide; U549S98QLW; 5-((3S,5R,8R,9S,10S,13R,14S,17R)-3,14-dihydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2H-pyran-2-one; 3beta,14beta-dihydroxy-5beta-bufa-20,22-dienolide; …

Botanical Source: Bufonis Venenum

Key Research Findings

1. Cancer Resistance Studies

   • Inhibits resistance-induced EMT
   • Targets SRC-3/c-Myc pathway
   • Reduces drug resistance
   • Suppresses metastasis

2. Molecular Mechanisms

   • Down-regulates SRC-3 protein
   • Reduces c-Myc expression
   • Inhibits prometastatic effects
   • Modulates multiple pathways

Research Applications

1. Cancer Research

   • Drug resistance studies
   • EMT investigations
   • Metastasis research
   • Combination therapy

2. Pathway Analysis

   • SRC-3 signaling
   • c-Myc regulation
   • Angiogenesis studies
   • Cell survival mechanisms

Latest Developments (2024-2025)

• Demonstrated efficacy in refractory cancers
• Shows potential as chemosensitizer
• Multiple mechanism involvement
• Enhanced therapeutic effects

Storage & Handling

• Store at -20°C
• Protect from light
• Avoid repeated freeze-thaw cycles
• Stock solution guidelines provided
• 24-month stability under recommended conditions

Quality Assurance

• GMP-compliant manufacturing
• Rigorous quality control
• Complete analytical documentation
• Traceable lot numbers
• Stability monitoring program

Package Sizes

• 1mg
• 5mg
• 10mg
• 25mg
• Bulk quantities available

Key Research Areas

1. Drug Resistance

   • Chemoresistance mechanisms
   • EMT modulation
   • Metastasis inhibition
   • Combination strategies

2. Molecular Targeting

   • SRC-3 pathway
   • c-Myc signaling
   • Angiogenesis regulation
   • Cell death mechanisms

This premium-grade Bufalin is designed for research institutions and pharmaceutical companies advancing therapeutic development and scientific research.

For research and development purposes only. Not for human consumption or clinical use.