Description

Rubusoside is a naturally occurring diterpene glycoside extracted primarily from the leaves of Rubus suavissimus S. Lee (Chinese sweet tea, Tian Cha), a plant native to southern China and traditionally used as a natural sweetener and medicinal herb. BIORLAB offers high-purity rubusoside (≥98%) isolated through advanced extraction and purification technologies. This compound has gained significant attention for its potent sweetening properties (approximately 115 times sweeter than sucrose), solubilizing capabilities, and diverse pharmacological activities, making it valuable for food science research, pharmaceutical development, and natural product chemistry.

Technical Specifications

• Appearance: White to off-white crystalline powder
• Purity: ≥98% (HPLC)
• Melting Point: 208-210°C
• Optical Rotation: [α]D25 -32° (c 1.0, methanol)
• UV Absorption: λmax 210 nm in methanol
• Solubility: Highly soluble in water (~74.3 g/100 mL at 25°C); soluble in methanol, ethanol; poorly soluble in non-polar solvents
• pH Value: 5.5-7.0 (1% aqueous solution)
• Sweetness Index: Approximately 115 times sweeter than sucrose

IUPAC Name: [(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (1R,4S,5R,9S,10R,13S)-5,9-dimethyl-14-methylidene-13-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxytetracyclo[11.2.1.01,10.04,9]hexadecane-5-carboxylate

InChI: InChI=1S/C32H50O13/c1-15-11-31-9-5-18-29(2,7-4-8-30(18,3)28(41)44-26-24(39)22(37)20(35)16(12-33)42-26)19(31)6-10-32(15,14-31)45-27-25(40)23(38)21(36)17(13-34)43-27/h16-27,33-40H,1,4-14H2,2-3H3/t16-,17-,18+,19+,20-,21-,22+,23+,24-,25-,26+,27+,29-,30-,31-,32+/m1/s1

InChIKey: YWPVROCHNBYFTP-OSHKXICASA-N

Canonical SMILES: CC12CCCC(C1CCC34C2CCC(C3)(C(=C)C4)OC5C(C(C(C(O5)CO)O)O)O)(C)C(=O)OC6C(C(C(C(O6)CO)O)O)O

Isomeric SMILES: C[C@@]12CCC[C@@]([C@H]1CC[C@]34[C@H]2CC[C@](C3)(C(=C)C4)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O)(C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O

Synonyms: Rubusoside; 64849-39-4; TCV5K3M3GX; Kaur-16-en-18-oic acid, 13-(beta-D-glucopyranosyloxy)-, beta-D-glucopyranosyl ester, (4alpha)-; 1-O-[(8alpha,9beta,10alpha,13alpha)-13-(beta-D-glucopyranosyloxy)-18-oxokaur-16-en-18-yl]-beta-D-glucopyranose; UNII-TCV5K3M3GX; Rubusoside?; Kaur-16-en-18-oic acid, 13-(ss-D-glucopyranosyloxy)-, ss-D-glucopyranosyl ester, (4a)-; 1H-2,10a-Ethanophenanthrene, kaur-16-en-18-oic acid deriv.; Rubusoside; …

Botanical Source: Hydrangea strigosa Rehd.

Storage and Handling Requirements

• Store at -20°C in airtight containers
• Protect from light and moisture (hygroscopic)
• Allow container to reach room temperature before opening
• Stock solutions can be prepared in water, methanol, or ethanol (10-50 mg/ml recommended)
• For maximum stability, store solutions at -20°C
• Avoid repeated freeze-thaw cycles
• Stable for 24 months when properly stored
• Working solutions should be prepared fresh
• Hygroscopic nature requires careful handling in humid environments

Research Applications

Food Science Research

• Natural Sweetener Studies: Excellent model for investigating non-caloric sweetener properties and taste mechanisms
• Sweetness Modulation Research: Important for studying taste-modifying properties and bitter-masking effects
• Food Formulation Studies: Valuable for developing sugar-reduced food products with improved taste profiles
• Stability Investigations: Critical for understanding thermal and pH stability in various food matrices
• Synergistic Sweetener Research: Important for studying sweetener combinations and potentiation effects

Pharmaceutical Applications

• Solubilizing Agent Research: Demonstrates exceptional ability to enhance solubility of poorly water-soluble drugs
• Bioavailability Enhancement Studies: Important for investigating improved drug delivery systems
• Natural Excipient Development: Valuable as a potential excipient for pharmaceutical formulations
• Taste-Masking Research: Critical for developing palatable pediatric and geriatric formulations
• Self-Emulsifying Drug Delivery Systems: Important for studying novel drug delivery approaches

Biological Activity Research

• Anti-inflammatory Studies: Shows potential inhibitory effects on inflammatory pathways
• Antioxidant Investigations: Demonstrates free radical scavenging capabilities
• Anti-diabetic Research: Valuable for studying glucose metabolism and insulin sensitivity
• Hepatoprotective Studies: Shows potential protective effects against liver injury
• Anti-obesity Research: Important for investigating metabolic regulation

Pharmacological Properties

• Sweetness Mechanism: Activates sweet taste receptors (T1R2/T1R3) with high potency
• Solubilizing Effects: Enhances aqueous solubility of hydrophobic compounds through micelle formation
• Anti-inflammatory Activity: Inhibits pro-inflammatory cytokine production and NF-κB pathway activation
• Antioxidant Properties: Scavenges free radicals and reduces oxidative stress markers
• Hepatoprotective Effects: Demonstrates protection against chemical-induced hepatotoxicity
• Anti-diabetic Potential: May help regulate blood glucose levels and improve insulin sensitivity
• Anti-obesity Activity: May influence lipid metabolism and adipogenesis

Source and Extraction

• Primarily isolated from leaves of Rubus suavissimus S. Lee (Chinese sweet tea)
• Constitutes approximately 5-8% of leaf dry weight
• Extracted using environmentally responsible water-based systems
• Purified through multi-stage chromatographic processes
• Structure confirmed through comprehensive spectroscopic analysis
• Standardized extraction protocols ensuring batch consistency

Quality Assurance

BIORLAB’s rubusoside undergoes:

• Comprehensive HPLC analysis for purity determination (≥98%)
• Structure verification by 1H and 13C NMR spectroscopy
• Mass spectrometry confirmation
• UV-Vis spectral characterization
• Stereochemical configuration confirmation
• Water content analysis
• Heavy metal screening
• Microbial limit testing
• Pesticide residue analysis
• Certificate of Analysis with batch-specific data

Technical Advantages

• High Purity: ≥98% HPLC purity ensures reliable research results
• Enhanced Solubility: Superior solubility in water compared to other steviol glycosides
• Excellent Stability: Demonstrates good stability under various processing conditions
• Dual Functionality: Combines sweetening and solubilizing properties
• Clean Taste Profile: Less bitter aftertaste compared to many other natural sweeteners
• Compatible with Various Systems: Works well in diverse food matrices and pharmaceutical formulations

Research Value

• Reference Standard: Essential for analytical method development and phytochemical profiling
• Solubilizing Agent: Valuable tool for improving solubility of hydrophobic compounds
• Natural Sweetener Model: Important for studying structure-sweetness relationships
• Pharmacological Tool: Useful for investigating multiple biological activities
• Food Ingredient Prototype: Essential for developing natural, low-calorie sweetener systems

Technical Support Services

• Method development assistance
• Analytical reference standard
• Custom purification options
• Bulk quantities available
• Technical consultation

For research use only. Not for human consumption or clinical use. All BIORLAB natural compounds are intended solely for laboratory research applications.

For detailed specifications, bulk quantities, or technical support, please contact BIORLAB’s scientific team.